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[hal-05118079] Impact of the physiological stage on the bioaccessibility of α-tocopherol from fortified yogurts using dynamic in vitro digestion simulating young and old adults' gastrointestinal conditions
Age-related physiological decline negatively impacts nutrient digestion and absorption, making older adults more susceptible to diet-related diseases. Fortified and functional foods are promising strategies to enhance the delivery of nutraceuticals and bioactive compounds. However, understanding how these foods behave under age-specific gastrointestinal conditions is essential. Despite the relevance of in vitro gastrointestinal models, there is a lack of dynamic systems that accurately replicate the digestive physiology of older adults. This study employed the DIDGI® dynamic in vitro gastrointestinal model, following the INFOGEST guidelines, to simulate the digestive processes of both young and old adults. Fortified yogurts containing α-tocopherol encapsulated in oil-in-water nanoemulsions were tested to assess the impact of age-related digestive conditions on digestibility and release kinetics of α-tocopherol. The intestinal recovery of α-tocopherol was significantly higher in the young adult model (97.3 ± 5.9 %) compared to the older adult model (79.8 ± 5.2 %). Although bioaccessibility during the intestinal phase was comparable in both models (ranging from 60.54 ± 7.38 % to 78.90 ± 8.88 %), the overall estimated bioavailability was significantly greater in the young adult model (67.76 ± 7.15 %) than in the older adult model (57.59 ± 4.50 %). Distinct release kinetics were also observed between models, indicating that physiological stage significantly affects nutrient release and absorption. This study demonstrates that aging alters digestive function, impacting the bioavailability of encapsulated bioactives. These findings underscore the importance of developing age-specific fortified foods and digestion models to optimize nutritional support for older adults.
ano.nymous@ccsd.cnrs.fr.invalid (Jean Michel Fernandes) 18 Jun 2025
https://hal.inrae.fr/hal-05118079v1
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[hal-05118224] Functional and nutritional properties of fava bean ingredients processed by extrusion
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ano.nymous@ccsd.cnrs.fr.invalid (I. Jebalia) 18 Jun 2025
https://hal.inrae.fr/hal-05118224v1
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[hal-05155723] Exploring the dairy fouling process in miniaturized falling film Evaporators by microfluidics
Fouling remains an unsolved challenge in the dairy industry, affecting operational efficiency and product quality. While extensive research has focused on fouling dynamics in heat exchangers, falling-film evaporators (FFE) have received less attention. There is scientific consensus that heat-induced protein denaturation is the main reason for fouling development, neglecting other potential mechanisms especially at relatively low temperatures. In a recent study [1], we demonstrated that increasing shear rates foster the formation of whey protein depos-its and enhance their structural complexity even at temperatures below the denaturation one. While highlight-ing the role of shear rate, this work does not shed light on the surface and bulk mechanisms leading to depos-it formation. In this context, we characterized in real-time the stages of dairy fouling development using microfluidic devic-es simulating FFE conditions. We aimed at discriminating the effects of shear (0-200 s-1) and temperature (50-75 °C) on the kinetics of deposit growth during the flow of whey protein solutions (10 wt. %). The key findings of this analysis can be summarized in two points: 1.At low (50 °C) and high temperatures (75 °C), no deposit formation and fast channel clogging are ob-served, respectively, thus underlining the strong influence of heat-induced protein denaturation. 2.At intermediate temperatures (65 °C), shear is a limiting factor controlling the accumulation of solids at the surface. These results provide a new insight on fouling mechanisms at the micron-scale and underline the coupled effect of shear and temperature under the typical FFE operational range of temperatures.
ano.nymous@ccsd.cnrs.fr.invalid (Margot Grostete) 10 Jul 2025
https://hal.inrae.fr/hal-05155723v1
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[hal-05151591] Towards a disruption in the manufacture of extremely low-cost micro-resonators using additive technologies and 3D printers outside the cleanroom : Story plus Laboratory visit
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ano.nymous@ccsd.cnrs.fr.invalid (Bruno Bêche) 08 Jul 2025
https://hal.science/hal-05151591v1
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[hal-05143864] SEAMPL, Un outil d'aide à la conception de scénarios de réemploi basé sur des critères environnementaux.
Le réemploi du verre fait partie des solutions prometteuses pour réduire les impacts environnementaux des emballages dans le secteur des boissons. Des potentiels de réductions des impacts sont souvent avancés pour les systèmes de réemploi, en comparaison de l’usage unique. Cependant, derrière ces potentiels optimaux se cachent une diversité de systèmes, d’organisations et donc de potentiels réels, dépendant de paramètres variables (taux de retour des bouteilles, taux de casse, poids, distances logistiques, etc.). L’Analyse du Cycle de Vie (ACV) est une méthode idéale pour quantifier les impacts de ces systèmes et pour chercher à les améliorer. Cependant, devant la complexité de la méthode, ainsi que son coût de mise en oeuvre, les acteurs peinent à en faire usage, en particulier en début de projet, reléguant son utilisation à une phase plus propice (le système est déjà sur de bons rails, la stabilité économique assurée), mais tardive. Cette utilisation tardive réduit la capacité de l’ACV à aider à l’écoconception des systèmes et la cantonne alors à un simple rôle de validation. Dans le cadre du projet européen FAIRCHAIN (2020-2024), le logiciel SEAMPL a été développé, pour permettre aux acteurs, non-spécialistes de l’évaluation environnementale, d’appliquer l’ACV à leur système en développement et d’inclure les aspects environnementaux dans le choix de différentes des orientations possibles. L’évaluation par ACV d’un système de réemploi nécessite de nombreux paramètres (estimés de 60 à 90 dans le cadre de ce travail). SEAMPL offre la possibilité de ne renseigner qu’un nombre réduit de ces paramètres tout en préservant la robustesse scientifique des résultats. Il se base pour cela sur les modèles ACV simplifiés paramétrés, par analyse globale de la variance. Les paramètres d’entrée retenus pour mesurer l’impact environnemental sont ceux contribuant à plus de 5% de la variance du résultat d’impact pour au moins une des seize catégories d’impact mesurées. Afin de couvrir une large gamme de systèmes de réemploi possibles, une typologie a été développée avec des acteurs du réemploi. Puis, des modèles simplifiés ont été développé pour chaque élément de la typologie. Ainsi SEAMPL fournit aux utilisateurs des modèles ACV types, et permet de simplifier grandement le travail de collecte de données. Ainsi, ils peuvent intégrer la dimension environnementale au plus tôt dans leur développement pour tester un grand nombre de scénarios envisageables (par exemple, comparaison d’un système dans lequel l’acteur aurait sa propre laveuse, avec un système dans lequel il sous-traiterait le lavage à un autre acteur, etc.).
ano.nymous@ccsd.cnrs.fr.invalid (Samuel Le Féon) 04 Jul 2025
https://hal.inrae.fr/hal-05143864v1
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[hal-05127077] Nutrition of the neonate: do differences in composition and structure between human milk and infant formula affect their digestion?
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ano.nymous@ccsd.cnrs.fr.invalid (Didier Dupont) 24 Jun 2025
https://hal.inrae.fr/hal-05127077v1
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[hal-05104637] DETERMINATION OF CALCIUM BIOACCESSIBILITY IN FOOD SOURCE OF DIETARY CALCIUM
Calcium is an essential nutrient required for biological functions. The recommended daily intake according to the WHO is 1000 mg of calcium per day for adults. However, calcium deficiency is widespread worldwide. Half of the world's population does not have adequate access to dietary calcium, particularly in diets with low dairy consumption. In addition, we are moving towards a healthier and more sustainable diet where it is recommended to increase the consumption of plant-based foods and reduce animal products (including dairy). This work aimed to study the bioaccessibility of calcium of 12 food sources of dietary calcium (plant food sources and dairy products), using an in vitro static gastrointestinal digestion model (INFOGEST). Calcium bioaccessibility was determined by the soluble fraction relative to total calcium present in the matrix. Soluble calcium content was measured at different time point of the gastric and intestinal digestion using inductively coupled optical emission spectrometry. During in vitro digestion, it was observed that the pH influenced the solubility of calcium for all the food matrices tested. Calcium is almost completely solubilized during the gastric phase at pH 3. During the intestinal phase, calcium solubility decreases considerably due to the increase of pH to 7. The presence of fat apparently affects calcium solubility in our model, probably though the formation of insoluble complexes in the intestinal phase. Indeed, matrices with higher fat content have a lower calcium solubility in the intestinal phase. A yogurt made from partially skimmed milk (1 g fat /100 g of food) has 0,52 g/L of soluble calcium whereas semi skimmed milk (1,6 g fat /100 g of food) has only 0,31 g/L. In addition, the best bioaccessibility at the end of the intestinal phase was observed for partially skimmed milk yogurt (34% ±0) followed by broccoli (27%± 1) and cheese (19%± 2). Calcium enriched plant-based drinks seem closer to semi-skimmed milk (21%±3 vs 26%± 2, respectively). On the other hand, fresh cheese, chickpea, whole almond, soy dessert and plant-based cheese-like product have very low calcium bioaccessibility (≤6%). If the ingested portion is considered to calculate calcium bioaccessibilities, dairy products (cheese, milk and yoghurt) are the best source of bioaccessible calcium. Indeed, ingesting a portion of yogurt (125 ml) represents 64.8mg of bioaccessible calcium compared to a portion of broccoli (80 g) which represents only 13.5mg of calcium. Thus, calcium is more bioaccessible in dairy products than in plant food sources.
ano.nymous@ccsd.cnrs.fr.invalid (Rozenn Le Foll) 10 Jun 2025
https://hal.inrae.fr/hal-05104637v1
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[hal-05144193] Ultrasound imaging of artificial tongues during compression and shearing of food gels on a biomimetic testing bench
Characterizing the deformations undergone by the tongue during food oral processing could help to better understand how texture sensations are perceived. In this article, we propose to study the potential of ultrasound (US) imaging to monitor the deformations undergone by artificial tongues during compression and shear of agar food gels. Four polyvinyl alcohol cryogels were used as artificial tongues (two levels of roughness and two levels of stiffness), while three agar gels of different concentrations were considered as model foods. Throughout the experiments, US images were acquired from a transducer array positioned underneath the artificial tongue, while force signals were obtained from a multi-axes sensor located above an artificial palate plate. Image analysis first consisted of tracing the contour of the dorsal surface of the artificial tongue. It was thus possible to observe how the deformations are distributed between the artificial tongues and the agar gels and to follow over time the heterogeneity of this distribution along the axis of the transducer array. Then, Particle Image Velocimetry (PIV) analysis was conducted to characterize the velocity fields related to deformations within the artificial tongue. In particular, the horizontal component of the velocity was studied during the shear movements and allowed one to distinguish static and dynamic friction phases, and to highlight the deformation gradients in the bulk of the artificial tongue. Such US method can provide a better understanding of the impact of the mechanical properties of food gels on the stimulation of mechanoreceptors responsible for translating mechanical stimuli into sensory perceptions.<p>This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. </p>
ano.nymous@ccsd.cnrs.fr.invalid (Miodrag Glumac) 04 Jul 2025
https://hal.science/hal-05144193v1
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[hal-04584647] Isolation, Identification, and Characterization of Bacillus cereus Group Bacteria Isolated from the Dairy Farm Environment and Raw Milk in Tunisia
Members of the Bacillus cereus group are well-known opportunistic foodborne pathogens. In this study, the prevalence, hemolytic activity, antimicrobial resistance profile, virulence factor genes, genetic diversity by enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) genotyping, and adhesion potential were investigated in isolates from a Tunisian dairy farm environment and raw milk. A total of 200 samples, including bedding, feces, feed, liquid manure, and raw bovine milk, were examined. Based on PCR test targeting sspE gene, 59 isolates were detected. The prevalence of B. cereus group isolates in bedding, feces, liquid manure, feed, and raw milk was 48%, 37.8%, 20%, 17.1%, and 12.5%, respectively. Out of the tested strains, 81.4% showed β-hemolytic on blood agar plates. An antimicrobial resistance test against 11 antibiotics showed that more than 50% of the isolates were resistant to ampicillin and novobiocin, while a high sensitivity to other antibiotics tested was observed in most isolates. The distribution of enterotoxigenic genes showed that 8.5% and 67.8% of isolates carried hblABCD and nheABC, respectively. In addition, the detection rate of cytotoxin K (cytk), enterotoxin T (bceT), and ces genes was 72.9%, 64.4%, and 5.1%, respectively. ERIC-PCR fingerprinting genotype analysis allowed discriminating 40 different profiles. The adhesion potential of B. cereus group on stainless steel showed that all isolates were able to adhere at various levels, from 1.5 ± 0.3 to 5.1 ± 0.1 log colony-forming unit (CFU)/cm2 for vegetative cells and from 2.6 ± 0.4 to 5.7 ± 0.3 log CFU/cm2 for spores. An important finding of the study is useful for updating the knowledge of the contamination status of B. cereus group in Tunisia, at the dairy farm level.
ano.nymous@ccsd.cnrs.fr.invalid (Randa Ben Akacha) 23 May 2024
https://hal.inrae.fr/hal-04584647v1
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[hal-05074488] Les protéines végétales: un nouvel eldorado?
Ce cours de 2h est adressé au public étudiant et professionnel intéressé par la question de la végétalisation de l'alimentation. Il s'inscrit dans un module d'enseignement sur la techno-fonctionnalité des protéines alimentaires et vise à interroger les opportunités mais aussi les verrous de cette nouvelle stratégie d'innovation.
ano.nymous@ccsd.cnrs.fr.invalid (Valérie Gagnaire) 20 May 2025
https://hal.science/hal-05074488v1
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[hal-05078889] Characterisation of aged infant formula powder based on ingredients produced by membrane filtration
The global infant formula (IF) market is expanding rapidly, driven by the increasing working women population, which limits breastfeeding opportunities. Spray-dried powdered IFs are widely used for their enhanced stability and reduced storage and transport. However, physicochemical changes such as the Maillard reaction, lactose crystallization, and fat oxidation during storage can compromise IF quality. Understanding these changes is essential for optimizing production and storage conditions, especially for minimally processed IFs designed to preserve nutritional integrity, for which the literature is scarce. Hence, we aimed at evaluating the physicochemical stability of IFs during storage. Three IF powders were produced at the semi-industrial scale: T-, without heat treatment; T+, with pasteurization of the concentrate alone (75°C, 2 min); T+++, combining milk pasteurization (72°C, 30 s) and heat treatment of the concentrate (85°C, 2 min) before spray-drying. They were then packaged in sealed metal cans under modified atmosphere and stored at room temperature and in a heat chamber at 40°C for periods of up to 11 and 6 months, respectively. Physicochemical properties, including moisture content, water activity, total fat and free fat content, whey protein denaturation were monitored. Maillard reaction was specifically assessed by monitoring lactosylation of whey proteins, and furosine and Nε-carboxymethyl-lysine content and color. All IF powders demonstrated satisfactory microbiological stability after 8 months at room temperature. No significant changes in moisture content or color were observed after 10 months at room temperature. Some variations were observed at room temperature for water activity, but it remained below 0.3. Protein denaturation was negligible, even after 6 months at 40°C. However, higher levels of lactosylation were detected across all IF powders, indicating the early stage of Maillard reaction took place despite low water activity. Thus, minimally processed IFs could achieve stability comparable to classically heat-treated ones, with lactosylation remaining as a challenge.
ano.nymous@ccsd.cnrs.fr.invalid (Xiaoxi Yu) 22 May 2025
https://institut-agro-rennes-angers.hal.science/hal-05078889v1
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[hal-05078242] Effect of transglutaminase on Maillard browning of lactose-free milk powder
Lactose intolerance is a clinical condition of the alimentary tract characterized by an individual's inability to digest and absorb lactose. This condition affects a non negligeable proportion of worldwide population. Thus, many dairy products have been developed by hydrolysing lactose. Hydrolysed milk in powder is not only challenging to produce due to problems related to caking, adherence to the chamber during spray drying and but also shows browning during storage. The application of enzymes such as transglutaminase (TGase) may have the potential to delay browning by rendering lysine unavailable, as it would be involved in the cross-linking process (Salunke &amp; Metzger, 2023; Chen et al., 2021; Francisquini et al., 2024). Thus, this study aims to evaluate the impact of TGase addition on the browning of lactose-hydrolysed powders. Different skimmed milk powders (control, with lactase, with TGase, with lactase and TGase) were produced at pilot scale and stored in controlled conditions (40 °C). They were sampled at different times during storage (0, 0.5, 1, 2 months) and analysed. The degree of polymerization (DP) of milk proteins was evaluated by size-exclusion chromatography. Targeted Maillard reaction markers (lactose, glucose, glucose as precursors and furfural, 5-methylfurfural, 2-furylmethylketone, furfuryl alcohol, 5-hydroxymethylfurfural as intermediate compounds) were quantified by chromatography. The degree of browning was determined by colorimetry. This approach makes it possible to explain the individual and synergistic role of precursors and enzymes together on reaction pathways. The extent of cross-linking of proteins by Tgase was confirmed and the hydrolysis of lactose into glucose and galactose was observed as expected. The results also show the apparent difference in the rates of precursors consumption and furanic compounds generation in different powders. These kinetic data enable not only a clearer understanding of the Maillard reaction in lactose-hydrolysed milk powders but could also be used for further kinetic modelling.
ano.nymous@ccsd.cnrs.fr.invalid (Carolina Neves Cunha) 22 May 2025
https://institut-agro-rennes-angers.hal.science/hal-05078242v1
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[hal-05101062] Application of Transglutaminase and Soluble Fiber in the Production of Lactose-Hydrolyzed Milk Powders
Dried dairy products containing hydrolyzed lactose pose significant technological challenges during spray drying, including reduced solubility, increased stickiness, and enhanced Maillard reaction (MR). This study evaluated the effects of transglutaminase (TGase) and soluble fiber on the physicochemical and structural properties of hydrolyzed lactose whole milk powder, aiming to mitigate these limitations. Six formulations of whole milk powder were developed. The addition of TGase resulted in increased particle size. Lactose hydrolysis intensified the MR, as evidenced by higher levels of 5-hydroxymethylfurfural (HMF) and increased yellowness in the hydrolyzed samples. Notably, TGase reduced HMF formation in the lactase-treated samples, suggesting a potential inhibitory effect on the MR post-processing. Microstructural analysis revealed particle agglomeration in the hydrolyzed samples and larger particle diameters in treatments containing TGase. Moreover, TGase application led to increased protein polymerization, as demonstrated by the presence of high-molecular-weight bands on electrophoretic profiles, indicating protein cross-linking. After five months of storage at ambient temperature, samples containing TGase exhibited lower HMF content and reduced browning intensity. Accelerated storage at 50°C further intensified browning in hydrolyzed formulations. However, TGase and fiber were not effective in preventing browning under elevated storage temperatures. Overall, the incorporation of TGase and soluble fiber appears to offer promising strategies to improve the stability and quality of hydrolyzed lactose milk powders, though further research is warranted to optimize their functional performance. This study is part of the international research project CAPES-COFECUB, conducted through a collaborative partnership between the Federal University of Juiz de Fora (UFJF), Brazil, and STLO, INRAe, Institut Agro, Rennes, France.
ano.nymous@ccsd.cnrs.fr.invalid (Jaqueline Dede Almeida Celestino) 06 Jun 2025
https://institut-agro-rennes-angers.hal.science/hal-05101062v1
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[hal-05111764] Modelling of the concentration and drying steps for skim milk powder production using machine learning approaches
In the current context of natural resource depletion and global warming, process efficiency has become a major challenge for dairy manufacturers, and optimization through modeling and simulation is one way of achieving it. For the world production of dairy powders (12.7 million tons in 2023 (CNIEL. 2024)), vacuum concentration and drying are the main steps but their modelling is complex due to the lack of knowledge of mechanisms involved in the process operations, the complexity of raw material and the multiple process schemes and equipment configurations depending on the type of powder produced. This study is part of a wider project which aims to optimize the concentration and drying steps for skim milk powder production. The main objective is to minimize energy consumption of these steps keeping constant powder quality regarding water activity, density and dispersibility. Moreover, modelling employs artificial intelligence, specifically machine learning algorithms, which use computational and algorithmic methods to develop data-driven predictors, offering new insights to understand complex relationships in the food industry (Mavani et al., 2022). 48 skim milk powders were produced at semi-industrial scale using a 2-effect falling-film evaporator and a 1-stage dryer. Physico-chemical and functional properties of concentrates and powders were determined. Subsequently, various machine learning algorithms were tested to address different optimization objectives. Promising results were obtained for the concentration step. Our model predicted the concentration factor from vapour pressure, skim milk dry matter content and feed flow rate (R2=0,90 with a random forest regressor). Addition of experimental data produced during the concentration of various dairy products (whey…) broadened the value range of operating conditions and strengthened the model. The next steps of this work are i) to reinforce the model by integrating more experimental data and considering other variables like concentrate viscosity; ii) to develop predictive models for the drying step. Cniel, 2024. L'Economie laitière en chiffres - Edition 2024. Centre National Interprofessionnel de l’Industrie Laitière, Paris.
ano.nymous@ccsd.cnrs.fr.invalid (Emeline Goussé) 13 Jun 2025
https://hal.inrae.fr/hal-05111764v1
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[hal-05078993] Use of transglutaminase for the production of lactose hydrolyzed milk powders
For population with lactose maldigestion, many dairy products with hydrolyzed lactose have been developed. However, spray drying of lactose hydrolyzed milk powder has challenges such as adhesion to the surface of drying chamber, reduced solubility and a tendency for caking (Torres et al., 2017). The use of transglutaminase (TGase) has shown modified techno-functionalities of several dairy products. Moreover, TGase treatment was found to improve flowing behavior and an increase in the transition temperature (Er et al., 2019) of skim milk powder. In our study, we explored the potential of TGase to address challenges related to lactose hydrolyzed milk powder. Four different skim milks (control, lactase-added, TGase-added, lactase and TGase-added) were concentrated 40% dry matter content and spray-dried at the pilot scale (n=3). Key characteristics such as the product adhesion to the equipment, the biochemical composition, particle size distribution and the presence of Maillard reaction markers were determined. Electrophoresis showed a decrease in the intensity of the casein bands in TGase-added powder, confirming glutamine-lysine crosslinking by TGase.. Our findings shows that the use of Tgase.did not affect the dryability of the milk but increased the particle size of the powders without altering their bimodal conformation. TGase did not affect the hydrolysis of lactose nor the color of the powders. However, the content of 5-hydroxymethylfurfural increased significantly in TGase-added powder suggesting different Maillard reaction pathways compared to the other powders. Thus, this study leads to new hypotheses and possibilities for future research to elucidate the influence of TGase addition on Maillard reaction mechanisms. Moreover, investigating the functionality of the powders such as rehydration properties could provide more insights into the potential application of TGase.
ano.nymous@ccsd.cnrs.fr.invalid (Júlia D’almeida Francisquini) 22 May 2025
https://institut-agro-rennes-angers.hal.science/hal-05078993v1
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[hal-05111680] Influence of drying parameters on the density of skim milk powder
The quality of a dairy powder is evaluated through a variety of properties, depending on its end use application, such as water activity, bulk density and rehydration properties, which are of primary importance. Controlling the density of dairy powders is crucial for manufacturers as it directly impacts production efficiency and packaging costs. However, although density is a key functionality, there are few studies in the literature focusing on it, more especially on the production parameters that influence the density of spray dried powders. Experimental data that evidence the effects of operating parameters on density are scarce. The objective of this work was to investigate the effects of operating factors for the production of skim milk powder on some powder functionalities such as apparent density, particle size distribution and flowability. The parameters studied were the conditions of liquid milk thermal treatment, the dry matter content of the concentrate at the inlet of dryer and the drying conditions (air flow rate, spray pressure, air inlet temperature). 48 trials of skim milk powder production were conducted at semi-industrial scale yielding robust results that evidenced some relationships between variables. Our findings showed that (i) interstitial air was significantly higher for concentrates with lower dry matter content, (ii) larger span led to higher apparent and tapped densities, and (iii) in trials with fine powder recycling and increasing dry matter content of concentrate, the span remained relatively unchanged, but mean particle diameter decreased. All these experimental results form a database which is currently used to develop models for predicting powder characteristics. Artificial intelligence, specifically machine learning is used to build these models. Promising results were already obtained for the prediction of the concentration factor during the vacuum evaporation step (results presented in another abstract**). ** Goussé et al. Modelling of the concentration and drying steps for skim milk powder production using machine learning approaches
ano.nymous@ccsd.cnrs.fr.invalid (Emeline Goussé) 13 Jun 2025
https://hal.inrae.fr/hal-05111680v1
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[hal-05079430] The Food Matrix drives the mechanisms of Oro-Gastrointestinal Digestion and the nutrient bioavailability
The structure of the food matrix is one of the key factors controlling the fate of food in the digestive tract and, consequently, the kinetics of nutrient release. The first part of this talk will review the state-of-the-art on the effect of food oral processing, in particular mastication, on the subsequent breakdown of food constituents in the stomach and the small intestine using bread, rice and other food structures as examples. Then, in a second part, using in vitro and in vivo digestion models (pigs), we will show that, for identical composition, differences in the macrostructure of dairy products (liquid vs. gel vs. solid) lead to differences in gastric emptying, protein hydrolysis in the intestine and amino acid bioavailability. When macrostructures are identical, differences at the microscopic level can also have a major impact on food digestion. For example, egg white gels produced by heat treatment at different pHs and ionic strengths show differences in digestion kinetics depending on the ability of pepsin to diffuse into the egg white gel structure. The structure of foods at different length scales can therefore be considered as a lever to control the kinetics of nutrient release during digestion and to meet the nutritional needs of specific populations (elderly, obese, athletes).
ano.nymous@ccsd.cnrs.fr.invalid (Didier Dupont) 22 May 2025
https://hal.inrae.fr/hal-05079430v1
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[hal-05070369] Pairing acidic and starch-rich foods lowers glycaemic responses by inhibiting oro-gastric starch hydrolysis: Evidence from in vitro semi-dynamic digestion
<div><p>Human studies over the past three decades have consistently shown that lowering the pH of starch-rich meals can significantly attenuate postprandial glycaemia. Based on our previous in vitro studies demonstrating the substantial contribution of salivary α-amylase during oro-gastric digestion of neutral-pH, starch-rich meals, we hypothesized that this effect is primarily mediated by the premature, acid-induced inhibition of salivary α-amylase during oral and/or gastric digestion. This study aimed to test this hypothesis by examining the starch digestion kinetics of food combinations from three independent human studies assessing the impact of combining acidic foods (white wine vinegar, pomegranate juice or kiwifruit) with bread or breakfast cereal. Five meals underwent semi-dynamic digestion in vitro using an adapted version of INFOGEST protocols. Samples were collected at all stages of digestion to determine the temporal profiles of starch release and hydrolysis into oligosaccharides. Approximately 40% of the total starch in bread was hydrolysed into oligosaccharides during orogastric digestion; however, this decreased to 15% -20% (&gt;50% reduction) with the addition of vinegar (p &lt; 0.01) or pomegranate juice (p &lt; 0.05). Similarly, for breakfast cereal, starch hydrolysis decreased from 35% to around 15% with kiwifruit (p &lt;0.001 to &lt;0.01). These results are consistent with the glycaemic attenuations reported in the literature for the same acidic meals, confirming our hypothesis and further substantiating existing knowledge on the relationships between meal pH, starch digestibility and glycaemic responses.</p></div>
ano.nymous@ccsd.cnrs.fr.invalid (Daniela Freitas) 16 May 2025
https://hal.inrae.fr/hal-05070369v1
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[hal-05059688] Revealing the dynamics and mechanisms of bacterial interactions in cheese production with metabolic modelling 6 may 2025 / Modélisation du microbiome
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ano.nymous@ccsd.cnrs.fr.invalid (David James Sherman) 07 May 2025
https://hal.inrae.fr/hal-05059688v1
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[hal-04998681] Starch digestion: A comprehensive update on the underlying modulation mechanisms and its in vitro assessment methodologies
Background: Starch is the predominant carbohydrate in our diets and its impact on human health is intricately linked with its digestive process. However, despite major advancements in the field, important inconsistencies in how starch digestion is described and studied in vitro still persist. Scope and approach: Our main objective was to provide up-to-date insights on starch digestion and fermentation in the human gastrointestinal tract, as well as related physiological responses and main drivers of variability (including inter-individual variations and structural and compositional differences between foods). A critical appraisal of digestion models and future work priorities is also presented. This work is the product of an international collaboration within the INFOGEST research network. Key findings and conclusions: Starch digestion is accomplished by the concerted action of luminal and brushborder hydrolases. Mechanical and biochemical transformations occurring throughout oral, gastric and intestinal phases can be determinant, contributing to different extents to the digestive process depending on food properties and individual characteristics. Numerous methodologies, with varying complexity and capacity to reproduce key digestive processes (e.g., gastric emptying), are currently available. Some in vitro digestibility outcome measures can be closely correlated with the outcomes of in vivo studies, demonstrating the promising potential of these approaches. However, the physiological relevance of in vitro starch digestion studies is often compromised by the lack of methodological consensus (particularly for oral digestion) and omission of key enzymatic events. Therefore, the need to develop harmonized guidelines and validate in vitro protocols adapted to starch digestion studies stand out as priority areas for future work.
ano.nymous@ccsd.cnrs.fr.invalid (Daniela Freitas) 20 Mar 2025
https://hal.inrae.fr/hal-04998681v1
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[hal-04862809] Drainage of acid-rennet gels: Rheological and microstructural study of milk heat treatment and milk proteins/whey protein aggregates ratio effects
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ano.nymous@ccsd.cnrs.fr.invalid (Marie-Hélène Famelart) 03 Jan 2025
https://hal.inrae.fr/hal-04862809v1
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[hal-05154008] Miniaturization of the fouling of whey proteins of Falling Film Evaporator by microfluidics
Fouling is a costly open question affecting the efficiency of unit operations and the quality of the products in dairy industry. The physico-chemical reasons leading to the accumulation of proteins and minerals on stainless-steel surfaces remain currently unclear. Little of the worksin the literature focused on the fouling dynamics in heat exchangers, leading to predictive models of fouling mechanisms based on the off-line analysis of solid deposits. Conversely, fouling has rarely been investigated in falling-film evaporators despite their systematic use in dairy industry (e.g., infant formula production). Up to now, fouling has been linked to heat-induced denaturation of the whey proteins. Other possible factors have been neglected despite deposit formation has been also observed at relatively low temperatures. In a recently published work [1], we have shown that shear stress has a significant impact on the formation of surficial deposits of whey proteins using a rheofluidic method. In particular, working at temperatures lower than the whey protein denaturation one (Td ≈ 70°C), we found that increasing shear rates (0-200 s-1) favored the increase of surficial deposits and the complexification of their structure, depending on protein concentration in the solutions. Following this study, we achieved an on-line observation of whey protein deposit formation using microfluidic devices mimicking the flow (100 s-1) and environmental conditions of falling-film evaporators (50-70 °C). We aimed at discriminating the role of shear and temperature on the kinetics of deposit growth during the flow of whey protein solutions with different concentrations (10-15 wt. %). Our observations revealed the simultaneous development of whey protein aggregates in the flowing solution and surficial deposits. Our results highlighted the impact of the difference of temperature between the solution and the microdevice on the development of the deposits rather than the residence time in the channels. A quantitative analysis of the evolution of protein aggregates in solution and of the kinetics of deposit surface occupation has been performed as a function of time and position in the channel.
ano.nymous@ccsd.cnrs.fr.invalid (Margot Grostete) 09 Jul 2025
https://hal.inrae.fr/hal-05154008v1
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[hal-05042512] Allocating environmental impacts between coproducts - Challenges of a methodological consensus
In most industrial food processes, fractionation is carried out to best valorized the different components of the raw material. For example, milk can be divided and processed into 5 co-products: cream, caseins, lactose and two whey fractions enriched respectively in α-lactalbumin and β-lactoglobulin. Assessing the environmental impacts of individual co-products addresses important issues, as the recovery of waste and ‘by-products’, the fair comparison of potentially substitutable products between two sectors, and the eco-design of assembly recipes. However, depending on the decisions taken to apply the Life Cycle Assessment (LCA) method, the environmental impacts attributed to each co-product can vary e.g. from 1 to 7 for cream, or even 1 to 10 for lactose. A methodological consensus is therefore needed on how to apply LCA to processed food products. Our work argues for using the most detailed description of the technological routes used, as over-simplified life cycle inventories lead to unrealistic attributions of impacts to irrelevant operations. The diversification of sensitivity analyses further helps to obtain the most useful LCA results for eco-design or comparative environmental footprint assessment.
ano.nymous@ccsd.cnrs.fr.invalid (Fanny Guyomarc'h) 22 Apr 2025
https://hal.science/hal-05042512v1
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[hal-05124696] Opinion and report of the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) on the assessment of the impact of fox population dynamics on public health
The Red Fox (Vulpes vulpes) can be hunted as a game species. It may also be listed as a “species likely to cause damage” (ESOD – the acronym in French), for public health reasons among others. Conversely, benefits linked to the presence of foxes are also put forward, such as the predation of rodents carrying zoonotic agents. In this context, ANSES was asked to: (1) list the zoonoses present in France for which foxes play an epidemiological role, (2) identify other public health impacts associated with changes in fox populations, (3) explain the relative importance of the effects of changes in fox populations for humans and/or the environment, (4) analyse the feasibility of a cost‐benefit analysis (CBA) of the prevention and impacts associated with these zoonoses. In France, the fox is a source of zoonotic pathogens, with a major role for Echinococcus multilocularis, a parasite for which the fox is the main source of environmental contamination. However, reducing fox populations does not reduce the risk of transmission of E. multilocularis to humans or domestic animals, and may even have the opposite effect to the one intended. The main levers for action are those relating to exposure to environmental contamination. The fox is part of complex trophic networks, in which its specific role in regulating prey populations is impossible to determine because (i) several predators share the same prey, with a variable role for the fox among the predators, (ii) the dynamics of prey populations is also conditioned by factors other than predation. The relationship between the abundance of rodents and the risk of disease for humans has not been demonstrated either, due to the complexity of the trophic and epidemiological networks, their highly probable variability from one ecosystem to another, and the multiplicity of hosts. As a result, the data currently available does not allow any conclusions to be drawn about the epidemiological role of the fox as a predator of rodents hosting zoonotic agents. Finally, there is no public health justification for culling foxes, particularly for ESOD classification, except in the very specific and localised context of the fight against bovine tuberculosis, for which the selective culling of foxes in and around the livestock buildings of a domestic outbreak has been proposed. In addition, the expertise concluded to the feasibility of a CBA targeted at assessing the burden of alveolar echinococcosis and measures to prevent its transmission. The feasibility study shows the partial nature of a CBA targeting zoonoses, without taking into account other roles played by the fox in the ecosystem.
ano.nymous@ccsd.cnrs.fr.invalid (Emmanuelle Gilot-Fromont) 23 Jun 2025
https://hal.science/hal-05124696v1
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[hal-05045657] Evidencing strain-dependency of metabolic pathways within 1,494 lactic bacteria genomes with the in silico screening Prolipipe pipeline
Genomes from bacteria of interest to the food industry exhibit significant functional variability, yet evaluating this characteristic remains challenging. As public repositories continue to accumulate more genomes, large-scale assessment of metabolic potential emerges as a promising method to highlight this functional variability. The primary challenge lies in automating a workflow to construct metabolic networks from genomes on a massive scale, with enzyme identification in sequences being a critical bottleneck. Here, we present Prolipipe, a pipeline designed for the large-scale assessment of metabolic potential in bacteria, focusing on specific pathways. Given a large dataset of hundreds to thousands of bacterial genomes with known taxonomy and a list of targeted pathways, Prolipipe identifies gene functions through a comprehensive annotation step using three different tools Then it builds genome-scale metabolic networks for each genome. These networks are then parsed to document the presence or absence of each reaction across all processed genomes and queried for reactions specific to particular pathways. By doing so, the pipeline evaluates the metabolic potential of each genome to carry out the pathway according to its gene content and highlight the best candidates among the large-scale set of genomes. In this study, Prolipipe was applied to 1,494 genomes of lactic acid bacteria, assessing the completion ratio of 761 pathways. We classified pathways according to their maximum completion rate, revealing that 137 pathways can be operated by at least one strain in our dataset. By mapping the identifiers of these pathways onto the pathway ontology graph of the Metacyc database, we highlighted that none of the pathways within four functional classes of Metacyc can be entirely recovered in the strain dataset. We then investigated infraspecific variability, a strong indicator of functional variability, and compared the species in our genome dataset based on their tendency to exhibit infraspecific variability. This analysis revealed species potential for strain-dependency, where phenotypes differ among strains of the same species -a feature observable in Prolipipe outputs.
ano.nymous@ccsd.cnrs.fr.invalid (Noé Robert) 06 May 2025
https://hal.science/hal-05045657v2
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[hal-04970111] Quantifying intestinal lipolysis with MRI and TD-NMR: Proof of concept using dairy cream digested in vitro
Understanding lipid digestion is crucial for promoting human health. Traditional methods for studying lipolysis face challenges in sample representativeness and pre-treatment, and cannot measure real-time lipolysis in vivo. Thus, non-invasive techniques like magnetic resonance imaging (MRI) need to be developed. This study assessed the MRI water-fat separation method for monitoring in vitro intestinal digestion of dairy cream, supported by high-performance thin-layer chromatography (HP-TLC) and time-domain nuclear magnetic resonance (TDNMR). A clear distinction was found between the T2 of undigested lipids (~120 ms) and lipolytic products (0.1–15 ms). The short T2 of lipolytic products likely results from semi-crystalline structures formed with bile salts. While MRI methods cannot detect such fast-relaxing protons, it effectively quantified lipolysis by tracking the residual undigested lipids, showing high correlation with HP-TLC results (R2 = 0.93 and 0.95 for 13-s and 6-min MRI methods, respectively). The rapid 13-s MRI method offers strong potential for future in vivo applications.
ano.nymous@ccsd.cnrs.fr.invalid (Ruoxuan Deng) 25 Apr 2025
https://hal.inrae.fr/hal-04970111v1
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[hal-05022863] Intraluminal enzymatic hydrolysis of API and lipid or polymeric excipients
The role of intraluminal enzymes for the hydrolysis of active pharmaceutical ingredients (API), prodrugs and pharmaceutical excipients will be reviewed. Carboxylesterases may hydrolyze ester-based API, prodrugs and ester-bond containing polymer excipients, whereas lipases digest lipid formulation excipients, such as mono-, di-and triglycerides. To clarify the conditions that should be mimicked when designing in vitro studies, we briefly review the upper gastrointestinal physiology and provide new data on the inter-individual variability of enzyme activities in human intestinal fluids. Afterwards, the methodology for studying enzymatic hydrolysis of API, prodrugs, lipid and polymeric excipients, as well as the main results that have been obtained, are summarized. In vitro digestion models used to characterize lipid formulations are well described, but data about the hydrolysis of lipid excipients (including surfactants) has been scarce and contradictory. Data on API and prodrug hydrolysis by esterases is available; however, inconsistent use of enzyme types and concentrations limits structure-stability relationships. Hydrolysis of polymer excipients in the lumen has not been significantly explored, with only qualitative data available for cellulose derivates, polyesters, starches, etc. Harmonization of the methodology is required in order to curate larger enzymatic hydrolysis datasets, which will enable mechanistic understanding and theoretical prediction.
ano.nymous@ccsd.cnrs.fr.invalid (Zahari Vinarov) 07 Apr 2025
https://hal.inrae.fr/hal-05022863v1
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[hal-05034901] chapitre 3 - Les yaourts et laits fermentés
Les produits laitiers fermentés sont appréciés pour leur qualité organoleptique, nutritionnelle et l’apport de bénéfices pour la santé. Parmi les produits laitiers reconnus de fort longue date, et au cœur des achats quotidiens d’une grande partie de la population, le yaourt est un produit fermenté qui continue à évoluer avec les nouvelles tendances de consommation, pour rechercher plus de naturalité et un plus grand respect de l’environnement. Cela se traduit également par des évolutions règlementaires sur les additifs et l’apparition de produits à base de différents végétaux, avec plus ou moins de succès.
ano.nymous@ccsd.cnrs.fr.invalid (Marie-Hélène Famelart) 15 Apr 2025
https://hal.science/hal-05034901v1
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[hal-05034852] Laits et produits laitiers quelques défis pour l’industrie laitière
Le lait, aliment complet consommé à tous les stades de la vie, est transformé en de nombreux ingrédients et produits laitiers, fermentés ou non, afin de le stabiliser et de le conserver. Ce livre présente les procédés de transformation innovants pour préserver la naturalité des composants laitiers et réduire leur empreinte environnementale. Différents défis auxquels est confrontée l’industrie laitière sont abordés concernant : les progrès pour développer des formules infantiles en phase avec les besoins physiologiques des nouveau-nés et les modèles de digestion in vitro associés ; les changements combinant tradition et nouvelles attentes des consommateurs pour réinventer le yaourt et les laits fermentés ; les pratiques optimales de fabrication fromagère pour contrôler à la fois la qualité du fromage et les performances de production ; les recherches actuelles pour répondre aux demandes des « consomm'acteurs » ; et la compréhension des mécanismes d'encrassement et la maîtrise du nettoyage des équipements de transformation pour limiter les consommations d'eau et d’énergie.
ano.nymous@ccsd.cnrs.fr.invalid (Valérie Gagnaire) 15 Apr 2025
https://hal.science/hal-05034852v1
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[hal-05015661] Exploring Bacillus velezensis in a biomedical context: a systematic review
<div><p>Bacillus velezensis is associated with producing secondary metabolites with antifungal, anti-inflammatory, antagonistic, and antimicrobial activities that have multiple applications in the food industry, agriculture, and farming. This study aims to review current knowledge to highlight the biomedical potential of Bacillus velezensis as a mammalian probiotic supported by in vivo and in vitro research and to compile existing knowledge. All information in this study was searched in the Dimensions, Scopus, PubMed, and Web of Science databases on 2023 Dec 10. The articles were selected according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method and scientometric analysis. A total of 1585 records were identified after the screening process. Of these, 151 were used for scientometric analysis, and 13 were used for data analysis based on in vitro and in vivo studies. The literature review revealed the great biomedical potential of B. velezensis and its relevance in scientific research, especially in recent years, thanks to advances in genome sequencing and the search for alternative antibiotics. The production of secondary metabolites such as surfactin, bacilysin, and fengycin is of growing interest to industry and human health. It also has great probiotic potential due to its antagonistic activity, lack of antibiotic resistance, and immunomodulatory activity. However, the optimization of its use and a better understanding of cultivation methods for gene expression under variable environmental conditions are crucial. Future research should focus on refining cultivation methods and applying techniques to maximize the production of these bioactive compounds to exploit the full potential of B. velezensis in various applications.</p></div>
ano.nymous@ccsd.cnrs.fr.invalid (Eduarda Guimarães Sousa) 01 Apr 2025
https://hal.science/hal-05015661v1
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[hal-05155673] Microfluidic investigation of fouling mechanisms in falling-film evaporators
Understanding fouling formation and preventing its consequences is of paramount importance in dairy industry in order to improve the efficiency of unit operations and the quality of the products. To date, most of the studies focused on the fouling dynamics considered the case study of heat exchangers, leading often to controversial even diametrically opposite results based on the off-line analysis of the deposits. Conversely, the fouling mechanisms have been rarely explored in falling film evaporators (FFE) despite their systematic use in the processing scheme of dairy powders. Moreover, their use in the production of high added value and sensitive products (e.g., infant milk formula) and the need to master water evaporation at this stage for environmental considerations make the understanding of FFE fouling even more crucial nowadays. In a previous work, we showed using an original rheometry approach that the initiation of the fouling of dairy products was not exclusively linked to protein thermal unfolding above the critical denaturation temperature, but was also based on the high shear rates in the vicinity to the equipment walls. Encouraged by these results, we performed here a direct observation at the microscale of the fouling growth by dairy mixes in a channel using microfluidic devices. The aim was to replicate the typical environmental and flow conditions of FFE in miniaturized models, providing direct in situ observations. Our results allow us to discriminate the mechanisms governing protein aggregation in the bulk solution and those occurring at the surface and leading to growing deposits.
ano.nymous@ccsd.cnrs.fr.invalid (Margot Grostete) 10 Jul 2025
https://hal.inrae.fr/hal-05155673v1
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[hal-05015653] The research on the identification, taxonomy, and comparative genomics analysis of nine Bacillus velezensis strains significantly contributes to microbiology, genetics, bioinformatics, and biotechnology
Introduction: Next-generation sequencing (NGS) has played a pivotal role in the advancement of taxonomics, allowing for the accurate identification, differentiation, and reclassification of several bacteria species. Bacillus velezensis is a Gram-positive, facultatively aerobic, spore-forming bacterium known for its antimicrobial and antifungal properties. Strains of this species are highly relevant in agriculture, biotechnology, the food industry, and biomedicine. Methods: In this study, we characterized the genomes of nine Bacillus strains isolated from soil in the state of Bahia (Brazil) using NGS with Illumina platform. Identification was performed by Average Nucleotide Identity (ANI) and digital DNADNA hybridization (dDDH) analyses, which revealed a match between the genomic information of the isolates and B. velezensis NRRL B-41580, with a variation of 89.3% to 91.8% by dDDH in TYGS and 95% to 98.04% by ANI in GTDBtk. Results and discussion: Two strains, BAC144 and BAC1273, exhibited high similarity to B. amyloliquefaciens subsp. plantarum FZB42. However, the latter strain was subsequently reclassified as B. velezensis. The division pattern observed during identification was confirmed in the phylogenomic analysis, where BAC144 and BAC1273 clustered with Bacillus amyloliquefaciens subsp. plantarum, while the other strains clustered with B. velezensis NRRL B-41580, forming a clade with high genetic similarity, with a bootstrap value of 100%. Furthermore, a synteny analysis demonstrated greater conservation mong the strains from this study compared to the reference strain, with the formation of distinct collinear groups. The pangenome analysis revealed an open pangenome, highlighting the genetic diversity within the species. Based on this analysis, a functional annotation was performed to compare exclusive gene repertoires across groups, uncovering distinct adaptations and functional profiles. The identification of bacterial strains belonging to this species is of great importance due to their high applicability. The strains identified in this study underscore the need for more robust taxonomic technologies to accurately classify prokaryotes, which are subject to constant evolutionary changes, requiring the reclassification of several species within the genus Bacillus, many of which are heterotypic synonyms of B. velezensis like Bacillus oryzicola, B. amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus.
ano.nymous@ccsd.cnrs.fr.invalid (Eduarda Guimarães Sousa) 01 Apr 2025
https://hal.science/hal-05015653v1
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[hal-04957546] Simulated gastrointestinal protein digestion of sheep and goat milk infant formulae
While cow and goat milks are used in manufacturing infant formulae (IFs), sheep milk is not allowed in European Union. To assess its suitability, we studied, by a peptidomics approach, the in vitro gastro-intestinal protein digestion of commercial sheep and goat IFs. In both IFs, after 120 min in the stomach, caseins were found massively degraded, while residual whey proteins were detected. In the intestine, β-lactoglobulin, and α-lactalbumin were found resistant to enzymes, particularly in sheep IFs. Compared to goat IF, sheep IF caseins showed a higher degradability, with higher number of released peptides and % protein coverage, particularly for κ-and α s1 -casein. On the contrary, whey proteins were found more hydrolysed in goat IF. In the intestine, for both IFs, the peptide profiles resembled those of the stomach, except for α-lactalbumin. Several bioactive peptides were identified, among these casein phosphopeptides. Overall, protein digestion parameters of sheep IF are comparable with goat IF.
ano.nymous@ccsd.cnrs.fr.invalid (Paola Scano) 19 Feb 2025
https://hal.inrae.fr/hal-04957546v1
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[hal-04863490] Viscoelastic and flow behaviour of β-lactoglobulin/lactoferrin coacervates: Influence of temperature and ionic strength
Heteroprotein complex coacervation has potential for a wide range of applications. However, the sensitivity of coacervates to slight changes in physico-chemical conditions may constitute a technological barrier for their development and deserves to be better understood. In this study, the rheological properties of β-lactoglobulin/lactoferrin (βLG/LF) heteroprotein complex coacervates were investigated with respect to narrow changes of temperature (5–40 °C) and ionic strength (0 to 10 mM added NaCl). The apparent viscosity of βLG/LF coacervates prepared at 20 °C showed a high sensitivity to temperature, decreasing progressively at elevated temperatures. Frequency sweep experiments demonstrated that coacervates behave as a viscoelastic liquid throughout the investigated frequency range at T &gt; 10°C. Time-temperature superposition principle revealed that the interaction involved in the coacervation process were temperature-independent. The calculated activation energy was approximately 85 kJ/mol. The addition of NaCl (up to 10mM) prior to coacervation, resulted in an increase of the viscosity but did not show a clear trend in the evolution of viscoelastic moduli. These new insights allow a better understanding of the interactions involved in concentrated protein coacervates enabling better control over their potential uses.
ano.nymous@ccsd.cnrs.fr.invalid (Rima Hachfi Soussi) 03 Jan 2025
https://hal.science/hal-04863490v1
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[hal-04971053] Protein–Protein Interactions and Structure of Heat-Set Gels Based on Pea Protein and Egg White Mixtures
The substitution of animal proteins with plant-based ones to fit environmental and economic demands is a challenge in gel applications. This study examined the thermal elation of mixtures of pea protein isolate (PPI) and egg white proteins (EWPs) at different PPI/EWP weight ratios (100/0, 75/25, 50/50, 25/75, 0/100) at pH 7.5 and 9.0. Viscoelastic and texture properties of the composite gels, along with the microstructure and molecular interactions involved in the gel network, were investigated. Except for PPI-EWP 100/0 at pH 9.0, all systems gelled with increasing gel hardness, springiness, and cohesiveness when EWP content increased. This was explained by the microstructure of the gels, wherein the presence of PPI enhanced the formation of aggregates embedded in the EWP network, thus loosening it. The rheological properties of the mixed gels were primarily influenced by the EWP network, involving disulfide bonds. However, upon the addition of PPI, hydrogen bonds and hydrophobic interactions predominated and the structure of the gel became more sensitive to pH as electrostatic repulsions interfered. Adjusting the ratio of PPI/EWP allows the production of gels with varying textures, and suggests the possibility of partially substituting egg white with pea proteins in food gel formulation.
ano.nymous@ccsd.cnrs.fr.invalid (Jian Kuang) 25 Mar 2025
https://hal.inrae.fr/hal-04971053v2
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[hal-04971213] Actualité UMR STLO - VEG&LAIT se mobilise dans les fermes participantes !
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ano.nymous@ccsd.cnrs.fr.invalid (Fanny Guyomarc'H) 28 Feb 2025
https://hal.science/hal-04971213v1
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[hal-04965794] The effects of Thymus capitatus essential oil topical application on milk quality: a systems biology approach
Essential oils (EO) are known for their antibacterial and anti-inflammatory properties and can be used as an alternative to reduce the reliance on antimicrobials in dairy cattle. While many studies have explored the beneficial properties of EO in vitro, their effects on milk quality and milk microbiota, when applied directly to the udder skin, remain relatively unknown. This study aimed to investigate the impact of Thymus capitatus essential oil (TCEO), known for its high antibacterial and antioxidant properties, on milk microbiota using 16S rRNA sequencing, the lipidomic profile via liquid chromatography-quadrupole time-of-flight mass spectrometry, udder skin microbiota, and inflammatory biomarkers of dairy cows at the end of lactation. Sixteen-quarters of 12 Holstein cows were selected, and TCEO was topically applied to the udder skin twice a day for 7 days. Milk was collected aseptically on days 0, 7, 21, and 28 before morning farm milking. The results showed no significant changes in microbiota composition after the EO treatment in alpha and beta diversity or taxonomical composition at the phylum and genus levels. TCEO induced limited changes in the milk lipidome, primarily affecting diacylglycerols at T21. The treatment did not affect inflammatory biomarkers, milk sensory properties, or quality. Our study is the first to demonstrate that a local application of 10% TCEO on cow’s quarters does not significantly alter milk quality or microbiota composition in milk and skin. More studies should be conducted to ensure the safe use of TCEO in dairy cows and explore its potential benefits on antibiotic-resistant bacteria as an alternative or support for antibiotic therapy.
ano.nymous@ccsd.cnrs.fr.invalid (Ralph Nehme) 28 May 2025
https://hal.inrae.fr/hal-04965794v1
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[hal-05093069] Production and application of inulinase by new isolates of Aspergillus welwitschiae from fermented peach-palm waste for the production of fructooligosaccharides
Microbial inulinase enzymes have a number of applications in biotechnology. In this study, new strains of Aspergillus welwitschiae were investigated as producers of inulinases and their endo-and exo-inulases were characterized in silico and their protein modeling was performed. The inulinase production by A. welwitschiae employing the Dohelert method to assess the interaction between temperature and cultivation humidity, resulted in a fourfold increase in activity. The optimal temperature and humidity were 25-27 • C and 75-82 %, respectively. The enzyme exhibited optimal activity at a pH of 3.5 and at 60 • C. The hydrolysis of the sisal root yielded 272 mg/g of reducing sugars and oligosaccharides. Our in silico experiments predicted 10 well-validated structures of endo-(5) and exo-inulinases (5) from A. welwitschiae. The notable activity of these inulinases on inulin highlights their potential for the treatment of agro-industrial residues with the objective of producing high-value added products.
ano.nymous@ccsd.cnrs.fr.invalid (Emanuelle Santos de Carvalho Cardoso) 02 Jun 2025
https://hal.inrae.fr/hal-05093069v1
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[hal-04970147] First assessments of nutrient bioaccessibility with an INFOGEST semi-dynamic gastric digestion in vitro protocol adapted to model proton pump inhibitor use
Physiologically relevant gastrointestinal digestion models for infants, adults, and the elderly are commonly used to explore the fate of food in vitro. However, no consensus protocol exists to simulate the specific conditions observed in the stomach of people using oral proton pump inhibitors (PPIs), a class of widely prescribed medications that reduce gastric acid secretion and may alter food digestion and nutrient absorption. The first objective of this study was to propose an in vitro gastric digestion protocol adapted to model PPI use. This protocol is an extension of the semi-dynamic INFOGEST protocol previously developed to mimic the digestion of an adult human (referred to hereinafter as “Standard”), with two modifications based on reported clinical effects of PPIs: (i) a final gastric pH of 4.2 and (ii) a 50 % reduction in simulated gastric acid fluid volume. The second objective was to compare the release kinetics of peptides, soluble carbohydrates, lipids, and minerals during simulated gastric digestion of a mixed meal (bread, cheese, and tomato) with both standard and PPI versions of the protocol. Results demonstrated that the release of peptides, arabinose, and minerals, including calcium, magnesium, and phosphorus, was significantly (p &lt; 0.05) reduced in the PPI model, while the hydrolyses of starch and lipids, assessed through maltose release and triacylglycerol disappearance, respectively, were not significantly affected. These findings are in agreement with the expected effects of reduced gastric acidity on pepsin activity and mineral solubility. These findings are also consistent with known or presumed side effects of PPIs such as an increased risk of hypomagnesemia, fractures, skeletal muscle loss, and vitamin B12 deficiency. In conclusion, this modified INFOGEST protocol appears to serve as a valuable tool to study the side effects of PPI use on food digestion and related nutrient bioaccessibility.
ano.nymous@ccsd.cnrs.fr.invalid (Ruoxuan Deng) 27 Feb 2025
https://hal.inrae.fr/hal-04970147v1
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[hal-04957552] Strategic Considerations in Designing Food Solutions for Seniors
The demographic shift towards an aged population calls for targeted nutrition strategies to support healthy aging and bridge the gap between life expectancy and a healthy life span. Older adults face various nutritional deficiencies, particularly in protein, vitamins (B12, D), minerals (calcium, iron), and dietary fiber. This work delves into the EAT4AGE project efforts that strategically aimed to develop age-oriented food products (European Joint Programming Initiative “A Healthy Diet for a Healthy Life” JPI HDHL). Currently, manufacturing of age-tailored food products presents significant complexities, from challenges of commercialization to the generation of acceptable and palatable food choices. As a first step, a literature-based comprehensive checklist has been developed to facilitate product development. This tool provides an integrated approach, ensuring that all critical aspects of product development are addressed systematically. Secondly, we describe the application of the tool in the development of a series of products, such as plant-based protein-rich cereals, reformulated dairy products, processed meat, and enriched spreads; all combining high nutritional values with adaptations to the physiological and sensory needs of seniors. Overall, this work offers insight into the current needs of seniors and a tool for product development that can be utilized for prospective product development, such as the ones detailed herein. Thus, the EAT4AGE hopes to set an example that will stimulate the fabrication of effective, well-received nutritional solutions, ultimately improving health outcomes for older adults.
ano.nymous@ccsd.cnrs.fr.invalid (Leehen Mashiah) 19 Feb 2025
https://hal.inrae.fr/hal-04957552v1
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[hal-04927524] Impact of ovotransferrin on the membrane integrity of Salmonella Enteritidis under egg-white conditions
Introduction: Eggs can mediate foodborne disease resulting in salmonellosis outbreaks that are most commonly caused by Salmonella enterica serovar Enteritidis. Ovotransferrin is a prominent egg-white antimicrobial glycoprotein belonging to the transferrin family, members of which exhibit powerful iron-chelating activity. However, several studies have also described the ability of transferrin proteins to disrupt bacterial membranes. This study aimed to investigate the antimicrobial activity of ovotransferrin toward S. Enteritidis membranes at 30°C under egg-white conditions.<p>Materials and methods: This aim was supported by the deployment of a synthetic medium designed to mimic egg-white (matching the ionic composition and pH). The ability of ovotransferrin to induce bacterial membrane permeabilization in S. Enteritidis was investigated by measuring substrate accessibility to periplasmic β-lactamase and cytosolic β-galactosidase.Results:The depolarization of the inner membrane of S. Enteritidis was measured using a fluorescence probe [DiSC 3 (5)]. The results show that ovotransferrin induces permeabilization of the outer membrane but not the inner membrane whereas egg white permeabilizes both membranes. In addition, the dissipation of the proton motive force by egg white was found to involve a contribution by ovotransferrin since this protein provoked inner-membrane depolarization. Discussion: It can thus be concluded that ovotransferrin exerts a membranes perturbation activity on S. Enteritidis under egg-white conditions, in addition to its well-known iron-chelation activity.</p>
ano.nymous@ccsd.cnrs.fr.invalid (Julie Legros) 03 Feb 2025
https://hal.science/hal-04927524v1
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[hal-04887772] Végétaliser notre alimentation : des légumineuses dans le yaourt
Comment diminuer l’apport de produits d’origine animale sans trop changer les habitudes alimentaires des consommateurs ? De nouvelles recherches visent à développer des yaourts en mélangeant du lait de vache et de la farine de lupin.
ano.nymous@ccsd.cnrs.fr.invalid (Valérie Gagnaire) 15 Jan 2025
https://hal.inrae.fr/hal-04887772v1
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[hal-04987925] Equine endometrial bacteria inhibition by metabolite and extracellular vesicles of Lactobacillus acidophilus and lactiplantibacillus plantarum
Endometritis is one of the main reproductive disorders in mares and due to the increasing prevalence of antibiotic resistance, the use of probiotics in the prevention and treatment of endometritis in mares has gained interest, given their potential to restore and maintain a healthy uterine microbiota. Therefore, the aim of this study was to evaluate the antimicrobial properties of total metabolites of Lactobacillus acidophilus (LA) and Lactiplantibacillus plantarum (LP) against common equine endometrial pathogenic bacteria in vitro (Acinetobacter baumannii, Escherichia coli (1), Escherichia coli (2), Escherichia coli (3), Escherichia coli (4), Enterobacter cloacae, Streptococcus equi, Staphylococcus warneri, Actinobacillus equi and Klebesiella pneumoniae), as well as to assess their low molecular weight metabolites (loM) and extracellular vesicle (EVs) inhibition capacity over a multidrug-resistant E. coli isolated from mares with clinical endometritis. The total metabolites of LA showed better inhibition on A. baumannii, E. coli (1) and E. cloacae, while those of LP inhibited E. coli (4), S. equi and A. equi. Besides total metabolites, loM of LA and LP can inhibit E. coli. LA EVs were more effective in preventing E. coli (2) compared to LA loM, while LP EVs presented inhibition but below 90%. The use of LA and LP in the mare's uterus may be an interesting approach to controlling endometritis. In addition to metabolites, EVs can contribute to the inhibition of multidrug-resistant E. coli.
ano.nymous@ccsd.cnrs.fr.invalid (Maria Clara Mendes Bernabe) 12 Mar 2025
https://hal.science/hal-04987925v1
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[hal-04891543] The protein ingredient quality of infant formula influences colonic physiology and microbiota and the serum metabolomic profile in neonatal mini-piglets
Despite the WHO recommendations in favor of breastfeeding, most infants receive infant formulas (IFs), which are complex matrices involving numerous ingredients and processing steps. Our aim was to understand the impact of the quality of the protein ingredient in IFs on gut microbiota and physiology, blood metabolites and brain gene expression. Three IFs were produced using whey proteins (WPs) from cheese whey (IF-A) or ideal whey (IFs-C and -D) and caseins, either in a micellar form (IFs-A and -C) or partly in a non-micellar form (IF-D). Twenty-four Yucatan minipiglets received one of the IFs from 2- to 21-days of age. The piglets were then euthanized 84 min postprandially. Blood, ileal and colonic digesta and tissues, the liver and the hypothalamus were sampled. Gut microbiota composition and activity, the expression of intestinal and brain genes involved in barrier, immune, endocrine, nutrient carrier and neuronal functions and serum metabolite levels were determined. Intestinal paracellular permeability was assessed with an ex vivo Ussing chamber. The data were analyzed using multifactorial and univariate analyses. The colon was the main site to be physiologically affected by the quality of the dairy protein ingredients used in IFs. Colonic paracellular permeability was significantly higher in IF-D-fed piglets than in those receiving IFs-A and -C, in line with the expression of genes encoding tight junction proteins (OCLN, CLDN3 and CLDN4). IF-D up-regulated the colonic expression of genes involved in the immune function (SOCS3, PIGR and TNFα) when compared to IF-A. Although intestinal α- and β-diversities did not significantly differ among IFs, some specific differences were observed, such as the abundances of Campylobacterota phylum and Bacteroides genera and fecal butyrate production, which were increased with IFs-C and -D versus IF-A. The kynurenine pathway was favored in piglets fed IF-D compared to IF-A, based on colonic gene expression and serum metabolites. In addition, levels of some serum metabolites, particularly putrescine, spermidine and spermine, were higher in piglets fed IF-D than IFs-A and -C. Overall, IF-D, which combined ideal WPs and some non-micellar caseins, appeared to differ most from IF-A in terms of its physiological consequences, suggesting that both WP and casein ingredient quality may mediate the physiological properties of IFs, probably through changes to the colonic microbiota composition and activity.
ano.nymous@ccsd.cnrs.fr.invalid (Lucile Chauvet) 16 Jan 2025
https://hal.science/hal-04891543v1
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[hal-04739412] Interaction between a Lactococcus lactis autochthonous strater and a raw goat milk microbial community during long term backslopping
Traditional cheesemaking processes often involve backslopping practice. However, over successive inoculations, acidification deficiencies may arise. In such cases, adding a starter is recommended to restore the ecosystem stability. This study examines the impact of an autochthonous starter composed of three Lactococcus lactis strains on a raw goat milk microbial community during their evolution. Bacterial composition and technological features (acidification and aroma) were analyzed during communities’ evolution over 800 generations. 16S rRNA gene metabarcoding showed that Lactococcus lactis strains predominated. The raw goat milk community acidification capacities varied early in the evolution and then remained stable. Adding the L. lactis starter to this community stabilized the ecosystem from the beginning of the evolution. The acetoin production was associated with the starter presence, consistent with the establishment of the diacetylatis biovar strain, from the starter in the raw goat milk community throughout the evolution. Increased or decreased production of some volatile organic compounds when the starter was added revealed a specific aroma footprint due to interactions between the two communities. This study showed that adding a starter could help to achieve the maximum acidification rate from the early inoculation cycles and could significantly modify the aroma profile during long-term backslopping.
ano.nymous@ccsd.cnrs.fr.invalid (Marie-Aurore Caillaud) 16 Oct 2024
https://hal.inrae.fr/hal-04739412v1
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[hal-04802584] Machine Learning for predicting industrial performance: example of the dry matter content of Emmental-type cheese
Controlling the dry matter content of cheese is essential to defining the performance of cheese production. For Emmental-type cheese, dry matter content has to be above but as close as possible to a minimal value that is defined by legislation. The means for achieving the target dry matter content was mostly left to the discretion of the cheese experts, who target a dry matter objective based on his expert knowledge and the deviation of cheese production. To date, the prediction of performance indicators, such as cheese dry matter content, can help cheesemakers to improve their production performance. Several Machine Learning models and classical statistical methods were compared to predict the dry matter of Emmental cheese for a set of data coming from one selected cheese industry. The Random Forest method emerged as the most effective model (RMSE = 0.28 and R² = 0.67). The weight of variables in explaining the variability of cheese dry matter content was also calculated, helping cheese experts to interpret the model and apply corrective actions to improve cheese production performance. The ability to predict cheese dry matter content and understand its variability from cheese manufacturing data offer new perspectives for the cheese industry. This method can be transferred to other indicators and assist in decision-making to enhance industry performance.
ano.nymous@ccsd.cnrs.fr.invalid (Manon Perrignon) 16 May 2025
https://hal.science/hal-04802584v2
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[hal-04683653] Peptidomic profile of human milk as influenced by fortification with different protein sources: An in vitro dynamic digestion simulation
Fortification of human milk (HM) is often necessary to meet the nutritional requirements of preterm infants. The present experiment aimed to establish whether the supplementation of HM with either an experimental donkey milk-derived fortifier containing whole donkey milk proteins, or with a commercial bovine milk-derived fortifier containing hydrolyzed bovine whey proteins, affects peptide release differently during digestion. The experiment was conducted using an in vitro dynamic system designed to simulate the preterm infant's digestion followed by digesta analysis by means of LC-MS-MS. The different fortifiers did not appear to influence the cumulative intensity of HM peptides. Fortification had a differential impact on the release of either donkey or bovine bioactive peptides. Donkey milk peptides showed antioxidant/ACE inhibitory activities, while bovine peptides showed opioid, dipeptil-and propyl endo-peptidase inhibitory and antimicrobial activity. A slight delay in peptide release from human lactoferrin and α-lactalbumin was observed when HM was supplemented with donkey milkderived fortifier.
ano.nymous@ccsd.cnrs.fr.invalid (Marzia Giribaldi) 02 Sep 2024
https://hal.inrae.fr/hal-04683653v1
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[hal-04937377] Dataset for common wheat (Triticum aestivum L.) grain and flour characterization using classical and advanced analyses
As global warming and changing market demand reshape agricultural practices, optimising the quality and utility of crop products, particularly wheat, is becoming increasingly complex and critical. Wheat plays a central role in human and animal nutrition, with its quality influenced by multiple factors at different scales, from grain composition to end-product performance, usually evaluated through sensory evaluation. Understanding the relationship between wheat composition and technological quality is essential for improving product value in agri-food systems. This dataset represents a broad panel of wheat samples encompassing diverse genetic backgrounds grown under varying environmental conditions in France. It collects measurements of grain, flour, dough and bread characteristics, facilitating a comprehensive comparison of wheat quality at different stages of production. The dataset encompasses 35 classical technological tests, 31 detailed compositional analyses—including in-depth characterization of protein composition (glutenin and gliadin), pentosan content measurement, and fatty acid profile analysis—and 37 sensory evaluations from the French Bread baking test providing detailed assessments of flour quality and dough behavior across key bread-making stages. In addition, raw data sets from Alveograph® and Farinograph® tests are included to support the development of innovative quality assessment criteria. This dataset will be valuable not only for the crop industry in its efforts to optimize wheat quality, but also for researchers and data scientists exploring the complex relationships between composition, processing and final bread quality. The data are registered in the French Research Data Gouv public repository and also stored in the PO2 Evagrain database using the PO2/TransformON ontology. The SPO2Q web tool allows for online database consultation, with further access available through the PO2 Manager desktop application.
ano.nymous@ccsd.cnrs.fr.invalid (Mélanie Munch) 10 Mar 2025
https://hal.inrae.fr/hal-04937377v2
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[hal-04830514] Strong electrostatic attraction drives milk heteroprotein complex coacervation
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ano.nymous@ccsd.cnrs.fr.invalid (Isabel Vinterbladh) 06 Jun 2025
https://hal.inrae.fr/hal-04830514v1
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[hal-04862413] Antibacterial effect of free, nanoemulsified and protein-complexed carvacrol
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ano.nymous@ccsd.cnrs.fr.invalid (Ralph Nehme) 03 Jan 2025
https://hal.inrae.fr/hal-04862413v1